I am supposing by the way is your question you are talkinga about multicellular organisms cell culture.
You should look out for medias used for the culture;
What kind of media you need for each type of culture(name of the media, proteins, sugar, salt, ph)?
Why is that usefull?
What type of tissues can be cultured?
Are there cells impossible to culture?
get all that and you should get a nice A+