No, it wont.
Even if it has bacteria in it right now, with no source of ammonia the bacteria will die, hence the cycle will die.
If you are 100% sure its a cycled tank you can keep the cycle alive by throwing in some fish food every other day as a source of ammonia.
I'm just curious, if it's an empty tank, how can you be so sure it has lots of bacteria in it?
The cycle doesn't start till u give a source of ammonia