uh oh... my brother decided to 'help' me clean my fish tank...?

i gave him the sponge to rinse in some of the old tank water i had already took out, but instead he went into the kitchen and washed in hot water. i think this would have killed the beneficial bacteria? can some body confirm this/ tell me what this would have done? i havent completely thrown everything off balance have i? or what will this do to the tank? how will this affect my fishes?
 
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