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Augusta STARZ

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STARZ is a group anybody can join! *All you have to do is add "STARZ" to the end of your name!

STARZ is for:

Some
Team
About
Raiding
Z-filled categories
(a way to say zzzz.... like sleeping)
 
ok, if you say so:




Effect of chemical modification of histidine and tyrosine residues in toxic shock syndrome toxin 1 on the serologic and mitogenic activities of the toxin.
N P Kokan-Moore and M S Bergdoll
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
This article has been cited by other articles in PMC.
Abstract
Modification of three or four of the five histidine residues in the toxic shock syndrome toxin 1 (TSST-1) with diethylpyrocarbonate did not inhibit the precipitin reaction of the modified TSST-1 with polyvalent antisera to the toxin. Monoclonal antibody 7T did not react with the modified TSST-1, but monoclonal antibody 8T did react with the toxin. Up to 50% of the mitogenic reaction of TSST-1 was inhibited by the histidine modification. Modification of one or two of the nine tyrosine residues in TSST-1 did not inhibit the precipitin reaction with polyclonal antisera to the toxin but did inhibit 85% of the mitogenic reaction.Full textFull text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (784K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

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1905 Selected ReferencesThese references are in PubMed. This may not be the complete list of references from this article. Bergdoll MS, Crass BA, Reiser RF, Robbins RN, Davis JP. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates. Lancet. 1981 May 9;1(8228):1017–1021. [PubMed]
Blomster-Hautamaa DA, Kreiswirth BN, Kornblum JS, Novick RP, Schlievert PM. The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. J Biol Chem. 1986 Nov 25;261(33):15783–15786. [PubMed]
Blomster-Hautamaa DA, Novick RP, Schlievert PM. Localization of biologic functions of toxic shock syndrome toxin-1 by use of monoclonal antibodies and cyanogen bromide-generated toxin fragments. J Immunol. 1986 Dec 1;137(11):3572–3576. [PubMed]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
Burstein Y, Walsh KA, Neurath H. Evidence of an essential histidine residue in thermolysin. Biochemistry. 1974 Jan 1;13(1):205–210. [PubMed]
Chang CT, Wu CS, Yang JT. Circular dichroic analysis of protein conformation: inclusion of the beta-turns. Anal Biochem. 1978 Nov;91(1):13–31. [PubMed]
Edwin C. Structure-function analysis of toxic shock syndrome toxin 1. Rev Infect Dis. 1989 Jan–Feb;11 Suppl 1:S137–S139. [PubMed]
Freed RC, Evenson ML, Reiser RF, Bergdoll MS. Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods. Appl Environ Microbiol. 1982 Dec;44(6):1349–1355. [PubMed]
Kokan-Moore NP, Bergdoll MS. Determination of biologically active region in toxic shock syndrome toxin 1. Rev Infect Dis. 1989 Jan–Feb;11 Suppl 1:S125–S129. [PubMed]
Miles EW. Modification of histidyl residues in proteins by diethylpyrocarbonate. Methods Enzymol. 1977;47:431–442. [PubMed]
Miller BA, Reiser RF, Bergdoll MS. Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent. Appl Environ Microbiol. 1978 Sep;36(3):421–426. [PubMed]
Reiser RF, Robbins RN, Khoe GP, Bergdoll MS. Purification and some physicochemical properties of toxic-shock toxin. Biochemistry. 1983 Aug 2;22(16):3907–3912. [PubMed]
Robbins R, Gould S, Bergdoll M. Detecting the enterotoxigenicity of Staphylococcus aureus strains. Appl Microbiol. 1974 Dec;28(6):946–950. [PubMed]
Schlievert PM, Shands KN, Dan BB, Schmid GP, Nishimura RD. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis. 1981 Apr;143(4):509–516. [PubMed]
Singh BR, Kokan-Moore NP, Bergdoll MS. Molecular topography of toxic shock syndrome toxin 1 as revealed by spectroscopic studies. Biochemistry. 1988 Nov 29;27(24):8730–8735. [PubMed]
Stelma GN, Jr, Bergdoll MS. Inactivation of staphylococcal enterotoxin A by chemical modification. Biochem Biophys Res Commun. 1982 Mar 15;105(1):121–126. [PubMed]
 
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STARZ is a group anybody can join! *All you have to do is add "STARZ" to the end of your name!

STARZ is for:

Some
Team
About
Raiding
Z-filled categories
(a way to say zzzz.... like sleeping)
 
Couldn't find the paste button, so I just unloaded my revolver into some swiss cheese.
The cheese was not innocent.

STARZ means
Some
Take
Answers
Religiously
(Zealots!)
 
A or B:
Red or Blue:
Tape or Glue:
Hello Kitty or Sonic the Hedgehog:
Cats or Dogs:
DOING THE Laundry or Dishes:
Sonic or Wendy's:
Wind or Rain:
ARE YOU Happy or Sad:
Friends or Family:
 
STARZ is a group anybody can join! *All you have to do is add "STARZ" to the end of your name!

STARZ is for:

Some
Team
About
Raiding
Z-filled categories
(a way to say zzzz.... like sleeping)
 
OMGLOLWTFBBQ is composed of 4 abbreviations (OMG, LOL, WTF, and BBQ) and was originally used to make fun of people who used abbreviations online a lot. It can carry the meaning of any or all of the constituent abbreviations.
 
Four, tres, two, uno

Listen up y'all ?cause this is it
The beat that I'm bangin' is delicious

Fergalicious definition
Make them boys go loco
They want my treasure
So they get their pleasures from my photo

You can see me, you can't squeeze me
I ain't easy, I ain't sleazy
I got reasons why I tease 'em
Boys just come and go like seasons

Fergalicious
(So delicious)
But I ain't promiscuous
And if you was suspicious
All that **** is fictitious

I blow kisses
(Mwah)
That puts them boys on rock, rock
And they be linin? down the block
Just to watch what I got
(Four, tres, two, uno)

So delicious
(It's hot, hot)
So delicious
(I put them boys on rock, rock)
So delicious
(They want a taste of what I got)
I?m Fergalicious
(T-t-tasty, tasty)

Fergalicious def-
Fergalicious def-
Fergalicious def-

Fergalicious definition
Make them boys go crazy
They always claim they know me
Comin' to me call me Stacy
(Hey Stacy)

I'm the F to the E, R, G, the I, the E
And can't no other lady put it down like me

I'm Fergalicious
(So delicious)
My body stay vicious
I be up in the gym
Just workin? on my fitness

He's my witness
(Ooh wee)
I put yo' boy on rock rock
And he be linin? down the block
Just to watch what I got
(Four, tres, two, uno)

So delicious
(It's hot, hot)
So delicious
(I put them boys on rock, rock)
So delicious
(They want a taste of what I got)
I?m Fergalicious
Hold hold hold hold hold up, check it out

Baby, baby, baby
If you really want me
Honey, get some patience
Maybe then you'll get a taste

Of my tasty, tasty
I'll be laced with lacey
It's so tasty, tasty
It'll make you crazy

T, to the A, to the S-T-E-Y, girl you tasty
T, to the A, to the S-T-E-Y, girl you tasty
D, to the E, to the L-I-C-I-O-U-S
To the D, to the E, to the, to the, to the
Hit it Fergie!

All the time I turn around brothas gather round
Always lookin? at me up and down, lookin? at my
(Uh)
I just wanna say it now I ain't tryin? to round up drama
Little mama I don't wanna take your man

And I know I'm comin? off just a little bit conceited
And I keep on repeatin? how the boys wanna eat it
But I'm tryin' to tell, that I can't be treated like clientele

?Cause they say she delicious
(So delicious)
But I ain't promiscuous
And if you was suspicious
All that **** is fictitious

I blow kisses
(Mwah)
That puts them boys on rock, rock
And they be linin? down the block
Just to watch what I got
Four, tres, two, uno

My body stay vicious
I be up in the gym
Just workin? on my fitness
He's my witness
(Ooh wee)

I put yo' boy on rock rock
And he be linin? down the block
Just to watch what I got
(Four, tres, two, uno)

So delicious
(Ay, ay, ay, ay)
So delicious
(Ay, ay, ay, ay)
So delicious
(Ay, ay, ay, ay)
I'm Fergalicious, t-t-tasty, tasty

So delicious
(Ay, ay, ay, ay)
So delicious
(Ay, ay, ay, ay)
So delicious
(Ay, ay, ay, ay)
I'm Fergalicious, t-t-t
(Ay, ay, ay, ay)

T, to the A, to the S-T-E-Y, girl you tasty
T, to the A, to the S-T-E-Y, girl you tasty
T, to the A, to the S-T-E-Y, girl you tasty
T, to the A, to the, to the, to the, to the
(Four, tres, two, uno)

To the D, to the E, to the L-I-C-I-O-U-S
To the D, to the E, to the L-I-C-I-O-U-S
To the D, to the E, to the L-I-C-I-O-U-S
To the D, to the E, to the, to the, to the, to the
(Four, tres, two, uno)

T, to the A, to the S-T-E-Y, girl you tasty
T, to the A, to the S-T-E-Y, girl you tasty
T, to the A, to the S-T-E-Y, girl you tasty
T, to the A, to the, to the, to the, to the
(Four, tres, two, uno)

To the D, to the E, to the L-I-C-I-O-U-S
To the D, to the E, to the L-I-C-I-O-U-S
To the D, to the E, to the L-I-C-I-O-U-S
To the D, to the E, to the, to the, to the, to the, to the
 
A or B:
Red or Blue:
Tape or Glue:
Hello Kitty or Sonic the Hedgehog:
Cats or Dogs:
DOING THE Laundry or Dishes:
Sonic or Wendy's:
Wind or Rain:
ARE YOU Happy or Sad:
Friends or Family:
 
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