Based on the diagram of the gel bands when doing a agarose gel electrophoresis experiment, please answer the two questions below.Please explain your answer briefly so I understand the concept.
THANKS
The diagram is in the following website: http://s687.photobucket.com/albums/vv231/Suchana6/?action=view¤t=img020.jpg
Questions:
1. Which sample has the smallest DNA fragment? Which sample has the largest? Do you need to run a KB ladder to know which sample has the smallest DNA fragment? Why or why not?
2. Assuming a circular piece of DNA (plasmid) was used as starting material, how many restriction sites were there in the DNA sample in lane 6? Now assume the plasmid used as starting material was linearized. How many restriction sites were there in the DNA sample in lane 6?
THANKS
The diagram is in the following website: http://s687.photobucket.com/albums/vv231/Suchana6/?action=view¤t=img020.jpg
Questions:
1. Which sample has the smallest DNA fragment? Which sample has the largest? Do you need to run a KB ladder to know which sample has the smallest DNA fragment? Why or why not?
2. Assuming a circular piece of DNA (plasmid) was used as starting material, how many restriction sites were there in the DNA sample in lane 6? Now assume the plasmid used as starting material was linearized. How many restriction sites were there in the DNA sample in lane 6?