Microbiology. How do you standardise a broth culture?

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jud_derman

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How do I make broth cultures from different strains of bacteria with approximately the same amount of bacteria in each container. In other word how do I standardise the viable count in a broth culture?

This is for a project I am doing testing the effectiveness of Various disinfectants on three known bacterial fish pathogens.

Thanks for your answers.
James.
 
The optical density in a spectrophotometer is proportional to the count for similarly sized bacteria. If it is two strains are comparably sized, they can be grow to the same O.D.
 
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