allele? This is a question from an exam that I recently took. Please explain the answer.
I want to design a diagnostic test to distinguish a mutant allele from a wild type allele, as in the two alleles depicted below. I have the choice of using two restriction enzymes (A and B) with target sites marked by the placement of A and B on the two alleles below. The resulting fragments from the digestion of the two alleles are indicated below in parentheses. Suppose you design a PCR test for detecting one or the other allele using a single set of primers. For your test, which of the following represent all valid choices of restriction enzymes to detect a difference between the two alleles?
__(100)__A__(300)__A__(100)___WT
____(500)_____B_____(500)_____Mutant
A. only A
B. only B
C. neither A nor B
D. A and B
E. you can only use A and B together
I want to design a diagnostic test to distinguish a mutant allele from a wild type allele, as in the two alleles depicted below. I have the choice of using two restriction enzymes (A and B) with target sites marked by the placement of A and B on the two alleles below. The resulting fragments from the digestion of the two alleles are indicated below in parentheses. Suppose you design a PCR test for detecting one or the other allele using a single set of primers. For your test, which of the following represent all valid choices of restriction enzymes to detect a difference between the two alleles?
__(100)__A__(300)__A__(100)___WT
____(500)_____B_____(500)_____Mutant
A. only A
B. only B
C. neither A nor B
D. A and B
E. you can only use A and B together