I didn't know it was 5 seconds so I kept it in 20 seconds because that's how long you keep a preg test in when you do the "cup way". Anyway, now I don't know if my tests will be accurate or not, I did 5 of them 20 seconds, 1 of them the right 5 seconds and I have 1 left to do. Does anyone know if it will affect the results or does it matter? Thanks in advance!