Did my kittens spray or pee on my daughter's princess dress? How can I tell?

Strong smell. Yellow spot on carpet. Daughter's dress reeked. I put it in the wash on hot water and bleach and got the smell out. My kittens are about 4 1/2 - 5 months old. Just got the boy fixed. I have seen the basement door (where the litter is kept) closed on certain occasions. You think they just didn't couldn't get downstairs. I have left the litter dirty for a day. You think this is why?

Was it even pee? If they sprayed, why? What can I do to stop it because if they continue, my husband will insist they find another home.
 
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