After the intitial conditioning process, Li-ion batteries can be topped up as often as is convenient for the user.
There's lots more great reference info on this site, a must read for all battery questions.
http://www.batteryuniversity.com/parttwo-34.htm
" A lithium-ion battery provides 300-500 discharge/charge cycles. The battery prefers a partial rather than a full discharge. Frequent full discharges should be avoided when possible. Instead, charge the battery more often or use a larger battery. There is no concern of memory when applying unscheduled charges.
Although lithium-ion is memory-free in terms of performance deterioration, batteries with fuel gauges exhibit what engineers refer to as "digital memory". Here is the reason: Short discharges with subsequent recharges do not provide the periodic calibration needed to synchronize the fuel gauge with the battery's state-of-charge. A deliberate full discharge and recharge every 30 charges corrects this problem. Letting the battery run down to the cut-off point in the equipment will do this. If ignored, the fuel gauge will become increasingly less accurate. (Read more in 'Choosing the right battery for portable computing', Part Two.)
Aging of lithium-ion is an issue that is often ignored. Depending on the state-of-charge and storage temperature, lithium-based batteries have a typical lifetime of 2-3 years (longer if partially charged and kept cool). The clock starts ticking as soon as the battery comes off the manufacturing line. The capacity loss manifests itself in increased internal resistance caused by oxidation. Eventually, the cell resistance will reach a point where the pack can no longer deliver the stored energy, although the battery may still contain ample charge. Increasing internal resistance is common to cobalt-based lithium-ion, a chemistry that is found in laptops and cell phones. The lower energy dense manganese-based lithium-ion, also known as spinel, maintains the internal resistance through its life but loses capacity due to chemical decompositions."
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