AP bio question..please help?

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Farrari_S

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A bacterial plasmid os 100 kb in length. the plasmid DNA was digested to completion with two restriction enzymes in three separate treatments: EcoRI, HaeIII, and EcoRI+HaeIII(double digest). the fragments were then separated with electrophoresis.
1. Describe how recombinant DNA technology could be used to insert a gene of interest into a bacterium?
2. How recombinant bacteria could be identified?
3. How expression of the gene of interest could be ensure?


Please help i will choose best answer for 10!
 
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