When I measure bilirubin levels with a spectrophotometer, why would I use HCl acid as a

C

Chamchell

New member
blank and not water? Normally I set my spectrophotometer to zero using distilled water only. However, with this experiment, measuring total bilirubin levels, I used hydrochloric acid+the bilirubin sample as a blank and then subtracted this from the results obtained from reading bilirubin sample+diazo reagent!

Is there reasoning behind this?

Thanks!
 
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