The hexapeptide gln-asp-lys-trp-cys-arg has pKas of 4.0, 4.3, 8.1, 9.0, 10.0, 12.2.
If this pentapeptide is hydrolyzed by chymotrypsin, what products will result? If these products are separated by phosphocellulose ion-exchange chromatography at pH 6, in what order will the products emerge from the column? Explain Why?
