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Three adjacent genes are involved in arginine biosynthesis. (They are structural genes of arginine operon.) You have three DNA fragments, each containing coding sequence for one of these three structural genes, and you have used these fragments to make three different probes for northern blotting (one probe for each of the three genes).
You have isolated mRNA from bacteria and you have performed the agarose gel electrophoresis followed by northern blotting.
What result do you expect to get if you use each of these three probes as a probe in northern analysis? What would be the result if three genes do not make up an operon?
Having trouble understanding the scenario. Any clarification would be helpful. Thanks!
You have isolated mRNA from bacteria and you have performed the agarose gel electrophoresis followed by northern blotting.
What result do you expect to get if you use each of these three probes as a probe in northern analysis? What would be the result if three genes do not make up an operon?
Having trouble understanding the scenario. Any clarification would be helpful. Thanks!