I need to prepare 4 % agarise gel for analysis of PCR products ( that will be used for microsatellite analysis in Urea - PAGE ). I dissolved 4 gm of agarose in 100 ml of 0.5 X TE buffer, the problem encountered is during solidification & mixing of ethidium bromide dye. The gel is too thick & cools very suddenly so can I mix the dye at initial stage , i mean just after heating & mixing of agarose ? The second thing is there are too many air bubbles & frothing during heating in oven ? how to remove them ?? Is there any special precautions for preparing gels of higher concentration ??
Preparation of 4 percent agarose for gel electrophoresis?
- Thread starter kush
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