A solution of the amino acid tyrosine was diluted by adding 2ml to 8ml or water.
When measured in the spectrophotometer in a 1cm path-length cuvette, this diluted solution had an absorbance of 0.95 at 294.5nm.
Given that the molar absorbance coefficient of tyrosine at 294.5nm is 2375 M-1 cm-1, calculate the concentration of the original, undiluted solution of tyrosine.
Please dumb this down for me and provide all calculations!
Thanks
When measured in the spectrophotometer in a 1cm path-length cuvette, this diluted solution had an absorbance of 0.95 at 294.5nm.
Given that the molar absorbance coefficient of tyrosine at 294.5nm is 2375 M-1 cm-1, calculate the concentration of the original, undiluted solution of tyrosine.
Please dumb this down for me and provide all calculations!
Thanks