I'm doing His purifications using the Qiagen Expressionist protocol and I see 2...

  • Thread starter Thread starter favoritegyrl1
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favoritegyrl1

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...bands with His antibody not 1? I've made several attempts to purify the protein and verify the presence of the protein with the His antibody but I keep getting two bands for each of the proteins. I really don't know what to do. Help!!!!
 
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