J
Joseba
Guest
We have a cup water of 100ml, if we add strong acid HCl to final concentration of 10mM.
a. What is the pH of the solution? (To simply, we ignore the addition of volume. And assume HCl is 100% dissociate in water).
b. When you dilute the buffer by 10 fold, what is the pH?
c. You make a buffer with HEPES at a final concentration to 0.1M. Then you adjust the pH to pH 7 (pKa ~ 7.5). What is the concentration of free acid and conjugated salt, respectively?
d. In the buffer you made in c, you add HCl to a final concentration of 10mM, what is the final pH after adding the acid?
a. What is the pH of the solution? (To simply, we ignore the addition of volume. And assume HCl is 100% dissociate in water).
b. When you dilute the buffer by 10 fold, what is the pH?
c. You make a buffer with HEPES at a final concentration to 0.1M. Then you adjust the pH to pH 7 (pKa ~ 7.5). What is the concentration of free acid and conjugated salt, respectively?
d. In the buffer you made in c, you add HCl to a final concentration of 10mM, what is the final pH after adding the acid?