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BT23
I adopted an unknown bacterium with a code number BT23 in the year 2010 of September from my beautiful and intelligent professor in Microbiology 1 class. The goal was to find the Genus name of my adopted unknown bacteria, and if fortunate enough, also find the species name. Throughout the semester I did different types of biochemical tests that might give a clue to what kind of bacteria BT23 really is. I wrote down all the results and observation on a safe and secure notebook so when I have done all the possible biochemical test, , I can easily look into it. These are what I got for my test:
Test
Observation
Test result
Motility test
The unknown spread in the motility agar.
Positive
Unknown is motile.
O2 requirement
Unknown grows more in air than without air.
Unknown is Facultative Anaerobic.
MacConkey’s Agar Plate
The plate turned from pink to beige. Colonies also grew.
Unknown is gram negative.
MacConkey’s inhibits gram positive bacteria.
Oxidase test
Karen did this test for us.
Negative
Unknown does not carry out respiratory metabolism.
Catalase test
Bubbles formed when with a drop of H202.
Positive
Unknown produces H202.
Lactose test
Broth did not change color. It was still red, but became turbid.
Negative
Unknown does not make acid or gas.
Glucose test
Broth turned from red to yellow. There were bubbles inside the glass tube.
Positive
Unknown is making acid and gas.
Nitrate test
The tube turned red
Positive
Unknown can reduce nitrate.
VP test
The broth did not change color.
Negative
Unknown does not produce butanediol.
MR test
Broth changed into light red color.
Positive
Unknown if positive for mixed acid fermentation.
Urea Hydrolysis
The orange agar became pink.
Positive
Unknown produces Urease.
Indole test
The broth had a red band on top.
Positive
Unknown has indole to degrade tryptophan.
Kligler’s Iron Deep
The agar had black stuff on the bottom.
Positive
Unknown produces hydrogen sulfide.
Test
Observation
Result
Simmon’s Citrate
The color was bluish-green.
It was hard to tell.
Gelatin test
It turned into gelatin like
Positive
Unknown makes the enzyme gelatinase.
I believe the most important test was to determine whether it is a gram positive or a gram negative bacterium, and then followed by shape. After I found out that BT23 was a gram negative rod, I looked into my notebook to see my biochemical test results. Not only did I found a Genus name, but also a species name. BT23 is called Proteus vulgaris.
I adopted an unknown bacterium with a code number BT23 in the year 2010 of September from my beautiful and intelligent professor in Microbiology 1 class. The goal was to find the Genus name of my adopted unknown bacteria, and if fortunate enough, also find the species name. Throughout the semester I did different types of biochemical tests that might give a clue to what kind of bacteria BT23 really is. I wrote down all the results and observation on a safe and secure notebook so when I have done all the possible biochemical test, , I can easily look into it. These are what I got for my test:
Test
Observation
Test result
Motility test
The unknown spread in the motility agar.
Positive
Unknown is motile.
O2 requirement
Unknown grows more in air than without air.
Unknown is Facultative Anaerobic.
MacConkey’s Agar Plate
The plate turned from pink to beige. Colonies also grew.
Unknown is gram negative.
MacConkey’s inhibits gram positive bacteria.
Oxidase test
Karen did this test for us.
Negative
Unknown does not carry out respiratory metabolism.
Catalase test
Bubbles formed when with a drop of H202.
Positive
Unknown produces H202.
Lactose test
Broth did not change color. It was still red, but became turbid.
Negative
Unknown does not make acid or gas.
Glucose test
Broth turned from red to yellow. There were bubbles inside the glass tube.
Positive
Unknown is making acid and gas.
Nitrate test
The tube turned red
Positive
Unknown can reduce nitrate.
VP test
The broth did not change color.
Negative
Unknown does not produce butanediol.
MR test
Broth changed into light red color.
Positive
Unknown if positive for mixed acid fermentation.
Urea Hydrolysis
The orange agar became pink.
Positive
Unknown produces Urease.
Indole test
The broth had a red band on top.
Positive
Unknown has indole to degrade tryptophan.
Kligler’s Iron Deep
The agar had black stuff on the bottom.
Positive
Unknown produces hydrogen sulfide.
Test
Observation
Result
Simmon’s Citrate
The color was bluish-green.
It was hard to tell.
Gelatin test
It turned into gelatin like
Positive
Unknown makes the enzyme gelatinase.
I believe the most important test was to determine whether it is a gram positive or a gram negative bacterium, and then followed by shape. After I found out that BT23 was a gram negative rod, I looked into my notebook to see my biochemical test results. Not only did I found a Genus name, but also a species name. BT23 is called Proteus vulgaris.