Hi to all,
I am facing problem during DNA sequencing. may be i am loosing my product during washing steps because the volume of cycle sequencing product is very small (20 microlt) so please detailed me cycle sequencing purification step.i am not using any kit for this step. i am using purification kit during PCR purification.so please help me for cycle sequencing purification step.
Thanks Chhavi
I am facing problem during DNA sequencing. may be i am loosing my product during washing steps because the volume of cycle sequencing product is very small (20 microlt) so please detailed me cycle sequencing purification step.i am not using any kit for this step. i am using purification kit during PCR purification.so please help me for cycle sequencing purification step.
Thanks Chhavi