genekelly_lover
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Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of
a.post-translational processing.
b.post-transcriptional processing.
c.restriction fragment ligation.
d.electroporation.
e.nucleic acid hybridization.
Which of the following is one of the technical reasons why gene therapy is problematic?
a.Most cells with an engineered gene do not produce gene product.
b.mRNA from transferred genes cannot be translated.
c.Cells with transferred genes are unlikely to replicate.
d.Transferred genes may not have appropriately controlled activity.
e.Most cells with engineered genes overwhelm other cells in a tissue.
To introduce a particular piece of DNA into an animal cell, such as that of a mouse, you would find more probable success with which of the following methods?
a.electroporation followed by recombination
b.the shotgun approach
c.transcription and translation
d.introducing a plasmid into the cell
e.infecting the mouse cell with a Ti plasmid
A researcher lyses a cell that contains nucleic acid molecules and capsomeres of tobacco mosaic virus (TMV). The cell contents are left in a covered test tube overnight. The next day this mixture is sprayed on tobacco plants. Which of the following would be expected to occur?
a.The plants would develop symptoms typically produced by viroids.
b.The plants would become infected, but the sap from these plants would be unable to infect other plants.
c.The plants would not show any disease symptoms.
d.The plants would develop the typical symptoms of TMV infection.
e.The plants would develop some but not all of the symptoms of the TMV infection.
a.post-translational processing.
b.post-transcriptional processing.
c.restriction fragment ligation.
d.electroporation.
e.nucleic acid hybridization.
Which of the following is one of the technical reasons why gene therapy is problematic?
a.Most cells with an engineered gene do not produce gene product.
b.mRNA from transferred genes cannot be translated.
c.Cells with transferred genes are unlikely to replicate.
d.Transferred genes may not have appropriately controlled activity.
e.Most cells with engineered genes overwhelm other cells in a tissue.
To introduce a particular piece of DNA into an animal cell, such as that of a mouse, you would find more probable success with which of the following methods?
a.electroporation followed by recombination
b.the shotgun approach
c.transcription and translation
d.introducing a plasmid into the cell
e.infecting the mouse cell with a Ti plasmid
A researcher lyses a cell that contains nucleic acid molecules and capsomeres of tobacco mosaic virus (TMV). The cell contents are left in a covered test tube overnight. The next day this mixture is sprayed on tobacco plants. Which of the following would be expected to occur?
a.The plants would develop symptoms typically produced by viroids.
b.The plants would become infected, but the sap from these plants would be unable to infect other plants.
c.The plants would not show any disease symptoms.
d.The plants would develop the typical symptoms of TMV infection.
e.The plants would develop some but not all of the symptoms of the TMV infection.