A
alina
Guest
Chemistry question--help!?
You need to prepare 25 ml of solution A for your experiment. According to the lab recipe, the composition of solution A is:
10mM Tris-Hcl pH 8.0
5mM EDTA
You found the following sterile stock solutions in the lab: one is 0.5 M Tris Hcl pH 8.0 solution, and the other is 0.1 M EDTA pH 8.0 There is also sterile water and bottles and you won't need to autoclave solution A.
To make 25ml of solution A, using the 0.5 M Tris Hcl pH 8.0 and 0.1 EDTA pH 8.0 solutions, you should add to the sterile bottle:
_____ml of the Tris Hcl solution
_____ml of EDTA stock solution.
Then, add sterile water to adjust the volume to 25ml.
Please show your work!
Thanks!!
You need to prepare 25 ml of solution A for your experiment. According to the lab recipe, the composition of solution A is:
10mM Tris-Hcl pH 8.0
5mM EDTA
You found the following sterile stock solutions in the lab: one is 0.5 M Tris Hcl pH 8.0 solution, and the other is 0.1 M EDTA pH 8.0 There is also sterile water and bottles and you won't need to autoclave solution A.
To make 25ml of solution A, using the 0.5 M Tris Hcl pH 8.0 and 0.1 EDTA pH 8.0 solutions, you should add to the sterile bottle:
_____ml of the Tris Hcl solution
_____ml of EDTA stock solution.
Then, add sterile water to adjust the volume to 25ml.
Please show your work!
Thanks!!