Here are the questions:
1. The tRNA used to prime the reverse transcriptase reaction during the conversion of the retroviral RNA genome to a double-stranded DNA genome
a. is encoded by the retrovirus
b. is also used during the transcription of the retroviral reverse transcriptase gene
c. is normally used during the translation of host-encoded protein-coding transcripts
d. synthesized using dNTPs
e. two of the above
2. In a standard PCR protocol, which is the correct order of steps in each cycle? a. annealing of the primers b. denaturation of the double-stranded DNA template c. extension by taq DNA polymerase d. removal of the primer by FEN-1
a. c, a, b, d
b. a, b, c
c. c, b, a, d
d. a, c , b, d
e. b, a, c
3. Which of the following does not involve gap repair?
1. insertion (i.e. "paste") of a DNA transposon
2. base excision repair
3. nucleotide excision repair
4. proofreading by DNA polymerase delta
5. two of the above
4. Reverse transcriptase has which common feature(s) with DNA polymerase alpha?
a. can only use DNA as a template
b. does not require a primer
c. incorporates dNTPs
d. synthesis occurs 3' to 5'
e. all of the above
5.Which of the following DNA repair mechanisms relies on differentiating the parental and daughter strands immediately after DNA replication?
1. error-prone double-stranded break repair by end joining
2. base excision repair
3. mismatch excision repair
4. nucleotide excision repair
5. two of the above
6.How many types of fluorescently-labeled reaction products will be produced in the following DNA sequencing reaction?
Reaction tube contains DNA polymerase, dNTPs (100 mM), fluorescently-labeled ddGTP (1 mM), primer (see below) and template (see below). DNA polymerase, primer and template are not rate limiting in this reaction (i.e., are present in excess in the reaction).
primer = 5'-CTTGGCTACTGCATTGGTC-3'
template = 5'-CCGTGATGCAATGGACCAATGCAGTAGCCAAGCTGCT-3'
Please answer with a number only.
7. Below is the terminal sequences of a DNA transposon. Please provide the nucleotide sequence indicated with X's and enter your answer written 5' to 3'.
5'- CACTAGGCTGCTGTA.................................XXXXXXXXXXXXXXX-3 '
3' -GTGATCCGACGACAT.................................YYYYYYYYYYYYYYY-5 '
8. During successful repair of double-strand DNA breaks by homologous recombination, how many potential outcomes (i.e. products) are possible though the alternative resolutions of the Holliday junctions?
Please answer with a number.
1. The tRNA used to prime the reverse transcriptase reaction during the conversion of the retroviral RNA genome to a double-stranded DNA genome
a. is encoded by the retrovirus
b. is also used during the transcription of the retroviral reverse transcriptase gene
c. is normally used during the translation of host-encoded protein-coding transcripts
d. synthesized using dNTPs
e. two of the above
2. In a standard PCR protocol, which is the correct order of steps in each cycle? a. annealing of the primers b. denaturation of the double-stranded DNA template c. extension by taq DNA polymerase d. removal of the primer by FEN-1
a. c, a, b, d
b. a, b, c
c. c, b, a, d
d. a, c , b, d
e. b, a, c
3. Which of the following does not involve gap repair?
1. insertion (i.e. "paste") of a DNA transposon
2. base excision repair
3. nucleotide excision repair
4. proofreading by DNA polymerase delta
5. two of the above
4. Reverse transcriptase has which common feature(s) with DNA polymerase alpha?
a. can only use DNA as a template
b. does not require a primer
c. incorporates dNTPs
d. synthesis occurs 3' to 5'
e. all of the above
5.Which of the following DNA repair mechanisms relies on differentiating the parental and daughter strands immediately after DNA replication?
1. error-prone double-stranded break repair by end joining
2. base excision repair
3. mismatch excision repair
4. nucleotide excision repair
5. two of the above
6.How many types of fluorescently-labeled reaction products will be produced in the following DNA sequencing reaction?
Reaction tube contains DNA polymerase, dNTPs (100 mM), fluorescently-labeled ddGTP (1 mM), primer (see below) and template (see below). DNA polymerase, primer and template are not rate limiting in this reaction (i.e., are present in excess in the reaction).
primer = 5'-CTTGGCTACTGCATTGGTC-3'
template = 5'-CCGTGATGCAATGGACCAATGCAGTAGCCAAGCTGCT-3'
Please answer with a number only.
7. Below is the terminal sequences of a DNA transposon. Please provide the nucleotide sequence indicated with X's and enter your answer written 5' to 3'.
5'- CACTAGGCTGCTGTA.................................XXXXXXXXXXXXXXX-3 '
3' -GTGATCCGACGACAT.................................YYYYYYYYYYYYYYY-5 '
8. During successful repair of double-strand DNA breaks by homologous recombination, how many potential outcomes (i.e. products) are possible though the alternative resolutions of the Holliday junctions?
Please answer with a number.